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Showing posts with label embryo. Show all posts
Showing posts with label embryo. Show all posts

Thursday, July 14, 2011

Mouse embryo anatomy





Mouse embryo (E12.5) dissection was performed so as to isolate and culture embryonic liver (last image). Liver appears red as during foetal development is the major site of erythropoiesis (i.e. the generation of blood cells). Third image shows the various developing internal organs of the GI tract at this point in the development of the embryo (i.e. after the outgrowth of the epithelium from the gut tube and the establishment of the various organ 'buds'). Images were taken from an iPhone 4 via a Leica stereomicroscope.

Tuesday, June 1, 2010

Immuno-histochemistry and In-situ Hybridization





Both pictures show mouse (mus musculus) embryos 9.5days old and 11.5d respectively.
In the top picture the embryo was hybridized using the anti-sense probe for FGF8 and detected by alkaline phosphatase conjugated antibodies which stained the specific sites of expression of FGF8. The areas in which the staining is more intense are the midbrain hindbrain boundary, forelimb bud and hindlimb bud, especially the edge of the hindlimb bud, the apical ectodermal ridge.
In the second picturethe embryo that was incubated with both primary antibodies and secondary conjugated with peroxidase antibodies, showing the specific sites of expression of neurofilaments. The visible staining represents neurofilaments, spinal ganglia (SpG) developing in the developing vertebrae and the developing cranial nerves, the vagus neurofilament, the glossopharyngeal (GlPh) neurofilament and the hypoglossal neurofilament.

Both pictures were taken from a camera connected to a dissection microscope.