Immuno-histochemistry and In-situ Hybridization

Both pictures show mouse (mus musculus) embryos 9.5days old and 11.5d respectively.
In the top picture the embryo was hybridized using the anti-sense probe for FGF8 and detected by alkaline phosphatase conjugated antibodies which stained the specific sites of expression of FGF8. The areas in which the staining is more intense are the midbrain hindbrain boundary, forelimb bud and hindlimb bud, especially the edge of the hindlimb bud, the apical ectodermal ridge.
In the second picturethe embryo that was incubated with both primary antibodies and secondary conjugated with peroxidase antibodies, showing the specific sites of expression of neurofilaments. The visible staining represents neurofilaments, spinal ganglia (SpG) developing in the developing vertebrae and the developing cranial nerves, the vagus neurofilament, the glossopharyngeal (GlPh) neurofilament and the hypoglossal neurofilament.

Both pictures were taken from a camera connected to a dissection microscope.